AS Level Biology - Factors that affect enzyme action

**This Powerpoint specifically uses the Cambridge AS Level Biology Syllabus and covers all criteria for – Unit 1.1 The microscope in cell studies
**
Students will be able to -

1. investigate and explain the effects of the following factors on
   the rate of enzyme-catalysed reactions:
   • temperature
   • pH (using buffer solutions)
   • enzyme concentration
   • substrate concentration
   • inhibitor concentration

2 explain that the maximum rate of reaction (Vmax) is used to
derive the Michaelis–Menten constant (Km), which is used to
compare the affinity of different enzymes for their substrates

3 explain the effects of reversible inhibitors, both competitive
and non-competitive, on enzyme activity

4 investigate the difference in activity between an enzyme
immobilised in alginate and the same enzyme free in solution,
and state the advantages of using immobilised enzymes

**Every Powerpoint includes learning objectives, a starter and plenary, suitable activities and review questions with mark scheme. We also provide a FREE pdf version of the file so it can easily be printed for students as a handout or uploaded to the school VLE system
**

Bring your Cambridge AS Level Biology lessons to life with our expertly designed teaching resources. Each unit is tailored to the latest syllabus, offering ready-to-use lesson plans, engaging experiments, and visually clear notes that save hours of prep time. Whether you’re new to the course or refining your approach, The Science Shelf gives you everything you need to boost student understanding and exam success — all in one place.