
Light-Driven Separation: Chromatography of Photosynthetic Pigments Pre-Lab Notes and Practical Guide
This chromatography pre-lab pack provides a clear, student-friendly introduction to the separation of plant pigments and the practical skills required for paper chromatography in an IB Biology context.
Keeping it Simple
This lab pack gives students a structured, accessible overview of chromatography theory and methods before they enter the laboratory. It uses clear text and audio support to build confidence, reinforce key ideas, and outline expectations with minimal overwhelming detail.
What’s Included in This Lab Pack
Student Pre-Lab Notes – A concise, engaging preparation resource with:
Clear explanation of chromatography principles and pigment separation
Visual diagrams of chromatography paper setup and solvent movement
Step-by-step walkthrough of the chromatography procedure
Guidance on identifying pigments and interpreting Rf values
Safety and handling notes for plant material and solvents
Sample calculations and examples of pigment separation analysis
Exam tips addressing common misconceptions about mobile and stationary phases
Teacher Notes – Practical guidance and classroom support:
Background theory linking chromatography to photosynthesis and pigment function
Suggestions for pre-lab discussion and concept checks
Common student difficulties (Rf misunderstanding, solvent choices)
Assessment opportunities related to data analysis and interpretation
Ideas for differentiation and extension activities
Technician Preparation Guide – Setup and materials checklist:
Materials list including chromatography paper, plant extracts, solvents, capillary tubes
Safety information for handling organic solvents and glassware
Preparation guidelines for leaf samples and solvent mixtures
Step-by-step setup instructions for chromatography stations
Troubleshooting tips for weak separations or solvent front issues
Audio Summary for Enhanced Learning
A podcast-style audio summary supports students who prefer auditory explanations.
Explains chromatography theory in simple conversational language
Walks through setup and common techniques
Highlights key safety considerations
Useful as pre-lab revision or homework preparation
Supports students who benefit from listening before practical work
Ideal For:
Students preparing for chromatography experiments
Audio learners who prefer spoken explanations
Teachers building practical prep materials
Technicians needing quick setup and safety guidance
This resource provides a straightforward pre-lab foundation for chromatography of photosynthetic pigments, helping students build confidence, practical skills, and readiness for independent laboratory work.
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