Negative Feedback Worksheet – Endocrine System (Thyroid Hormones)

This worksheet helps students understand negative feedback in the endocrine system, using the hypothalamus–pituitary–thyroid (HPT) axis as a clear, structured example.

Students label key structures and hormones involved in thyroid regulation, including TRH, TSH, and thyroid hormones, and identify stimulating vs inhibiting pathways. The activity reinforces how hormone levels are controlled to maintain homeostasis.

Suitable for KS3–KS4 Science, GCSE Biology, and senior secondary Biology / Human Biology.

What’s Included

Endocrine negative feedback labelling worksheet

Focus on the thyroid hormone feedback loop

Clear identification of:

Hypothalamus

Anterior pituitary

Thyroid gland

Target cells

TRH and TSH

Student worksheet + answer key

Printable, no-prep format

Learning Outcomes

Students will be able to:

Describe how negative feedback controls hormone levels

Identify the roles of the hypothalamus, pituitary, and thyroid

Explain how stimulation and inhibition regulate body systems

Apply understanding of homeostasis to endocrine regulation

Suitable For

KS3 & KS4 Science

GCSE Biology

Senior secondary Biology / Human Biology

Revision, homework, cover lessons, or assessment preparation

Format

PDF

Black-and-white friendly

Print or digital use