Making monoclonal antibodies lesson created in accordance to the NEW AQA Specification (9-1). Designed for a higher ability SEPARATE (trilogy) class, although content can be adjusted to suit any ability. Includes: slide animations, embedded videos and practice questions with answers on slides.
NB: This resource is for separate science only
AQA spec link: 126.96.36.199
Relevant chapter: B6 Preventing and treating diseases. AQA Biology third edition textbook-Page 106-107
Specification requires students to know the following;
Students should be able to describe how monoclonal antibodies are produced.
Monoclonal antibodies are produced from a single clone of cells. The antibodies are specific to one binding site on one protein antigen and so are able to target a specific chemical or specific cells in the body.
They are produced by stimulating mouse lymphocytes to make a particular antibody. The lymphocytes are combined with a particular kind of tumour cell to make a cell called a hybridoma cell. The hybridoma cell can both divide and make the antibody. Single hybridoma cells are cloned to produce many identical cells that all produce the same antibody. A large amount of the antibody can be collected and purified.